Paper describing Wuhan virus sequencing

A paper published in the New England Journal of Medicine entitled “A Novel Coronavirus from Patients with Pneumonia in China, 2019” describes how China CDC scientists took “lower respiratory tract samples…from patients with pneumonia of unknown cause” and “who had been present at the Huanan Seafood Market”.

“Extracted nucleic acid samples [RNA] were tested for viruses and bacteria by polymerase chain reaction” (PCR).  RNA extracted from lung fluid and culture supernatants was used as a template to clone and sequence the genome using genomic software.”  This paper references China’s PCR test.

“Primers were subsequently designed for PCR“.

“Although our study does not fulfill Koch’s postulates, our analyses provide evidence implicating 2019-nCoV in the Wuhan outbreak”.

The resultant genetic sequence that will become known as SARS-CoV-2.  A computer generated sequence of a virus not isolated and purified. [1, 2]